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KMID : 0603820160220030098
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Journal of Experimental & Biomedical Science
2016 Volume.22 No. 3 p.98 ~ p.106
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Performance of HPV E6/E7 mRNA Genotyping Test on Paired Cervical Cancer Exfoliated Cells and Formalin Fixed Paraffin Embedded Tissues
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Park Sun-Young
Wang Hye-Young
Kim Sung-Hyun
Kim Gee-Hyuk
Bong Sung-Young
Jang Hyoung-Soon
Park Sang-Jung
Hwang Koo-Yeon
Lee Dong-Sup
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Abstract
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Investigation of human papillomavirus (HPV) in archival formalin-fixed paraffin-embedded (FFPE) material is important for understanding cervical carcinogenesis. The objective of the present study was to identify the high risk HPVs (HR-HPVs) using HPV E6/E7 mRNA testing from archival tissues in cervical cancer and the relation to HR-HPVs genotypes in paired cervical exfoliated cells. HPV E6/E7 mRNA testing and DNA chip testing were performed in 79 paired cervical FFPE tissues and exfoliated cells from women with histologically confirmed squamous cell carcinoma and adenocarcinoma. Overall agreement in HR-HPVs detection from FFPE samples and cytology samples were 98.5% in HPV 16, 100% in HPV 18, HPV 31, HPV 33, HPV 58, HPV 66, and HPV 68. Type-specific agreement between FFPE samples and cytology samples was 89.1% in HPV positive, 93.5% in HPV 16 and more than 70% in the other HR-HPVs. In conclusion, HR-HPVs were reliably detected in paired FFPE and cytology samples with some variation in type-specific detection.
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KEYWORD
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Cervical cancer , Ki67 , HPV E6/E7 , RT-qPCR , High risk HPVs
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